MBI 414/514 - Immunology Principles
MBI 415/515 - Immunology Principles and Practice

Sample Questions for Exam 2

MULTIPLE CHOICE: Choose the letter corresponding to the ONE answer for each question.

1. Which statement about antigen processing is correct?
a. Exogenous antigens are processed via cytosolic fragmentation and presented on the cell surface in association with MHC-II molecules.
b. Proteosomes are important as chaperones that help position and stabilize MHC-I molecules on the inner surface of the endoplasmic reticulum as they are being assembled.
c. Endogenous antigens are processed via cytosolic fragmentation and presented on the cell surface in association with MHC-I molecules.

2. Which statement about generation of TCR diversity is correct?
a. To form a functional β-chain gene, both TCR α-gene and β-gene segments undergo gene rearrangement.
b. TCR diversity is determined solely by combinatorial joining of multiple germ-line V, D, J and C gene segments.
c. Sequences between the "chosen" TCR V-gene segment and the "chosen" J-gene segment are excised during the process of generating α-chain genes.

3. Which statement concerning generation of diversity in B cells is correct?
a. The so-called "N" diversity generated by mistakes made during Ig gene rearrangement during B cell maturation is important for antibody diversity.
b. TdT (terminal deoxynucleotidyl transferase) is not important in generating antibody diversity, especially in CDR3.
c. RAG1 and RAG2 are recombination activation genes, but do not appear to be associated with the recombinase activity needed for Ig gene rearrangement.

MATCHING MULTIPLE CHOICE: Choose the BEST answer. An answer may be used more than once within a set of questions.

Answers to questions 4-5:
a. TCR
b. MHC class II molecules
c. BCR
d. MHC class I molecules
e. more than one of these
4. ______ participate in the presentation of antigen peptides following partial antigen degradation in phagolysosomes.

5. Cell surface structures which recognize a peptide derived from a foreign protein antigen, together with a "self" molecule are _____.

TRUE FALSE: If a statement is TRUE, print T in the blank; if it is FALSE, print F.

6. Because primary TCR gene transcripts include both introns and exons, their processing to form functional mRNA includes removal of introns by SNRPs as well as capping and addition of a poly-A tail.

7. As demonstrated by Kruisbeek's experiment involving knockout mice, negative selection during T cell development involves recognition of MHC molecules, since MHC-1 knockout mice failed to develop CD4+ T cells and MHC-2 knockout mice failed to develop CD8+ T cells.

8. Using affinity labeling techniques, Wu and Kabat showed that the hypervariable regions of antibody molecules contain contact residues (amino acids that actually form noncovalent bonds with the antigenic determinants) and are therefore considered to be the CDRs.

ESSAY and/or PROBLEM-SOLVING:

9. Generate a diagram that depicts the fine details of MHC1 structure in the region that binds peptides, then explain how it facilitates MHC1-Ag binding.
 
10. Outline the classical pathway of complement activation, then discuss how it is negatively regulated.

11. Compare and contrast the manner in which B cells and T cells rearrange their receptor genes as they mature.

12. Compare and contrast the mechanisms used for positive and negative selection during development of B cells and T cells.


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© 1996-2014 John R. Stevenson. All Rights Reserved

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email questions and comments to:
John R. Stevenson, Ph.D.
Associate Professor
Department of Microbiology
Miami University
Oxford, Ohio 45056
USA
This document was last modified on Thursday, 09-Oct-2014 15:09:02 EDT